TOTAL NONDIALYZABLE SOLIDS (TNDS) IN HUMAN URINE. III. A METHOD FOR SUBFRACTIONATION OF RS-1 SOLIDS*

Open Access
Publisher Website
Google Scholar
Abstract
The nonultrafiltrable, veronal buffer-soluble fraction (RS-1) of total nondialyzable solids from normal human urine was subjected to the first steps of Cohn''s Method 10 for the separation of plasma proteins. This system of fractional precipitation employs low concentrations of ethanol and metal ions in aqueous solutions of low ionic strength, operating at low temperature with critical control of pH. Three distinct and apparently reproducible fractions were obtained and analyzed for total N, hexose, "sialic acid," hexosamine, hexuronic acid, sulfhydryl content, and lipids. The low isoelectric points, high glucide content and absence of sulfhydryl groups suggest that RS-1 consists almost entirely of plasma mucosubstances plus a small quantity of albumin.