Kinetic mechanism of NADH‐enoyl‐ACP reductase from Brassica napus
Open Access
- 31 October 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 484 (2) , 65-68
- https://doi.org/10.1016/s0014-5793(00)02128-1
Abstract
Enoyl‐ACP reductase, a component of fatty acid synthase, is a target for anti‐microbial agents and herbicides. Here we demonstrate the kinetic mechanism to be a compulsory‐order ternary complex with NADH binding before the acyl substrate. Matrix‐assisted laser desorption ionisation mass spectrometry analysis of enzymatically and synthesised crotonyl‐ACP substrate showed the former to contain a single acyl group, whereas the latter contained up to four additional crotonylations. The use of authentic crotonyl‐ACP will be important in future kinetic and crystallographic studies.Keywords
This publication has 22 references indexed in Scilit:
- The X-ray structure of Escherichia coli enoyl reductase with bound NAD + at 2.1 Å resolution 1 1Edited by R. HuberJournal of Molecular Biology, 1998
- Modification of the NADH of the Isoniazid Target (InhA) from Mycobacterium tuberculosisScience, 1998
- A Mechanism of Drug Action Revealed by Structural Studies of Enoyl ReductaseScience, 1996
- The Enoyl‐[Acyl‐Carrier‐Protein] Reductase (FabI) of Escherichia coli, which Catalyzes a Key Regulatory Step in Fatty Acid Biosynthesis, Accepts NADH and NADPH as Cofactors and is Inhibited by Palmitoyl‐CoAEuropean Journal of Biochemistry, 1996
- Common themes in redox chemistry emerge from the X-ray structure of oilseed rape (Brassica napus) enoyl acyl carrier protein reductaseStructure, 1995
- Crystallization of the NADH-specific Enoyl Acyl Carrier Protein Reductase from Brassica napusJournal of Molecular Biology, 1994
- inhA , a Gene Encoding a Target for Isoniazid and Ethionamide in Mycobacterium tuberculosisScience, 1994
- Induction, purification and characterization of NADH-specific enoyl acyl carrier protein reductase from developing seeds of oil seed rape (Brassica napus)Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- The Characteristics of Some Components of the Fatty Acid Synthetase System in the Plastids from the Mesocarp of Avocado (Persea americana) FruitEuropean Journal of Biochemistry, 1982
- The kinetics of enzyme-catalyzed reactions with two or more substrates or productsBiochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1963