CYTO-TOXIC AND CYTOKINETIC EFFECTS OF 1-BETA-D-ARABINOFURANOSYLCYTOSINE, DAUNORUBICIN, AND 6-THIOGUANINE ON HELA-CELLS IN CULTURE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (3) , 1127-1132
Abstract
Exponentially growing [human cervical carcinoma] HeLa cells were exposed to 1-.beta.-D-arabinofuranosylcytosine, daunorubicin and 6-thioguanine singly and in combination for 21 h. Survivals were determined by a colony-counting assay; cytokinetic effects of the drugs were evaluated by flow cytometry. When used in 2 drug combinations (concurrent exposures), 1-.beta.-D-arabinofuranosylcytosine protected cells against 6-thioguanine- and daunorubicin-induced lethality, the former to a greater extent than the latter. Protection against 6-thioguanine appeared related to the degree of inhibition of DNA synthesis by 1 -.beta.-D-arabinofuranosylcytosine, this was not the case with daunorubicin. When cells were exposed to the 3 drugs simultaneously, survivals were in agreement with those predicted from the various 2-drug combinations. Apparently no additional drug interactions occurred when a 3 drug was added to the 2-drug combinations.

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