Energy-filtered transmission electron microscopy of ferritin.
- 1 January 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (1) , 106-107
- https://doi.org/10.1073/pnas.79.1.106
Abstract
The focusing properties of a magnetic-sector spectrometer are shown to be suitable for forming high-spatial resolution, energy-filtered transmission electron microscope images. Filtered images of ferritin molecules by using electrons scattered from the characteristic iron M2,3 and carbon K absorption edges clearly distinguish the 75-.ANG. iron core and 120-.ANG. protein shell. The minimum detectable mass is estimated to be 0.84 .times. 10-20 g for Fe for an electron dose of 18 C/cm2 and 99% confidence.This publication has 12 references indexed in Scilit:
- Calcium release and ionic changes in the sarcoplasmic reticulum of tetanized muscle: an electron-probe study.The Journal of cell biology, 1981
- Parallel recording of electron energy loss spectraUltramicroscopy, 1981
- Correction of the second-order aberrations of uniform field magnetic sectorsUltramicroscopy, 1980
- Electron probe analysis of muscle and X-ray mapping of biological specimens with a field emission gun.1979
- The use of electron energy loss spectroscopy for studying membrane architecture: A preliminary reportUltramicroscopy, 1978
- The ultrastructure of ferritin macromoleculesJournal of Molecular Biology, 1978
- Formulae for light-element micro analysis by electron energy-loss spectrometryUltramicroscopy, 1978
- Electron probe x-ray analysis of single ferritin molecules.Proceedings of the National Academy of Sciences, 1976
- The microanalysis of light elements using transmitted energy loss electronsUltramicroscopy, 1975
- Electron MicrospectroscopyAnnual Review of Biophysics and Bioengineering, 1975