Polarographic Assay of Hydrogen Peroxide Accumulation in Microbial Cultures
- 1 January 1975
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 29 (2) , 170-174
- https://doi.org/10.1128/aem.29.2.170-174.1975
Abstract
A method is described for determining low concentrations of hydrogen peroxide by using a polarographic oxygen electrode to measure the oxygen released into solution on addition of catalase. A sample can be assayed directly without prior manipulation in 3 min. The method is capable of assaying hydrogen peroxide concentrations as low as 7 μM. The method has proved extremely useful for the assay of hydrogen peroxide secreted into milk by lactic acid bacteria. ImagesKeywords
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