Loss of blood platelet adhesion after heating native and cultured human subendothelium to 100 Celcius

Publisher Website
Google Scholar
Abstract
Study objective – Balloon angioplasty produces mechanical vessel wall injury that leads to substantial blood platelet deposition at the angioplasty site. The aim of the study was to determine whether thermal angioplasty might, by contrast, reduce platelet adhesion by denaturation of subendothelial adhesive proteins. Design – Native and cultured human subendothelium was briefly heated to ≥ 100°C by laser irradiation or to 50-100°C by immersion in preheated phosphate buffered saline (PBS). Subsequently, the subendothelium was exposed for 5 min to flowing human blood at a shear rate of 1300 s−1. Blood platelet adhesion to the subendothelium was determined quantitatively. Experimental material — Human umbilical arteries were used and the subendothelial matrix derived from cultured umbilical vein endothelial cells. Measurements and results – After heating arterial subendothelium by laser irradiation to ≥100°C, zero platelet adhesion was found v 36(SD 2)% adhesion to the non-heated surface (pv 31(7)% at 37°C (pConclusions – These in vitro results, if extrapolated to catheter interventions, suggest that thermal injury to the vessel wall by laser angioplasty or other thermal angioplasty methods may provide a basic and clinically relevant advantage over mechanical angioplasty modalities, because of a potentially reduced risk of complications related to platelet adhesion.

This publication has 1 reference indexed in Scilit: