Effects of Ethanol on Bicarbonate-Stimulated ATPase, ATP, and Cyclic AMP in Canine Gastric Mucosa

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Abstract
Previous reports have demonstrated that ethanol inhibits acid secretion and active transport in the gastric mucosa. In this study, enzymes which have been implicated in gastric active transport (Mg2+ and Mg2+ HCO3- ATPases), ATP and c[cyclic]AMP were evaluated in response to ethanol. The activities of Mg2+ and Mg2+HCO3--stimulated ATPases were not altered at concentrations below 10% ethanol, but were significantly inhibited with 15 and 20%. Tissue slice incubations with 20% ethanol show significantly decreased ATP concentrations at 10, 20 and 30 min. Exposure of the dog gastric mucosa in vivo to 20% ethanol produced a significant decrease in ATP content but did not alter the cAMP level. The ethanol-produced decrease in gastric ATP content may be involved in the inhibition of active ion transport observed with ethanol, but cAMP is probably not involved.