Characteristic immunophenotyping artefact seen in patients with anti-mouse immunoglobulin antibodies

Abstract
Patients treated with murine monoclonal antibodies develop anti‐mouse immunoglobulin antibodies which can interfere with flow cytometry of lymphocytes, giving rise to high levels of non‐specific staining. This artefact can be avoided by using separated, washed lymphocytes for immunophenotyping.