Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

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Abstract
Two-dimensional polyacrylamide gel electrophoresis and the radioligand (-)-[125I]iodopindolol (125I-Pin) have been used to study isoproterenol-depending protein phosphorylation and .beta.-adrenergic receptor availability, respectively, in cultured Sertoli cells and freshly isolated seminiferous tubular segments of sexually immature and mature rats. Sertoli cells prepared from sexually immature rats show progessive 125I-Pin binding in primary cultures that correlates with isoproterenol-induced cell shape changes, redistribution of immunoreactive vinmentin, and phosphorylation of this intermediate filament protein. The development of 12OI-Pin binding to Sertoli cell lysates is blocked by cycloheximide. Seminiferous tubules do not show significant isoproterenol-dependent vimentin phosphorylation nor 125I-Pin binding. However, vimentin phosphorylation can be induced by FSH or a cyclic nucleotide analog. The need for correlationg pharmacological-induced responses observed in Sertoli cell primary culturs with those in the intact seminiferous tubule is stressed.