DIRECT VISUALIZATION OF 10-NM (100-A)-FILAMENT NETWORK IN WHOLE AND ENUCLEATED CULTURED-CELLS

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 31  (JUN) , 393-409
Abstract
Following extraction of actomyosin and tubulin from Triton X-100-treated cultured cells, a cytoskeleton remains which is composed predominantly of the cell nucleus encompassed by a network of 10.0-nm filaments. In epithelioid [rat kangaroo] PtK2 cells this network is particularly prominent and contains a large proportion of 10-nm filaments associated in branched and meandering bundles. Enucleation of 3T3 cells with cytochalasin B gives rise to karyoplasts surrounded by 10-nm filaments. Concanavalin A had no marked effect on the distribution of the 10-nm-filament net in [mouse fibroblast] 3T3 cells. The 10-nm filaments probably act primarily as structural elements serving, in particular, to support and constrain the nucleus in its position in the cell.

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