Extended TIP(P) Analogues as Precursors for Labeled δ-Opioid Receptor Ligands

Publisher Website
Google Scholar
Abstract
Tyr-Tic-Phe-Phe-OH (TIPP) and the shorter Tyr-Tic-Phe-OH (TIP) peptides are potent and highly selective antagonists at the δ-opioid receptor and, therefore, are ideal candidates for the attachment of labels to assist in the study of δ-opioid receptors. Peptides extended at the C-terminus with residues which can be used as handles for further modification and/or labeling (i.e. Asx, Glx, and Lys) were synthesized. The TIPP-d/l-Asx/Glx derivatives exhibited similar δ-receptor affinity to TIPP (Ki = 5−10 nM vs Ki = 6 nM), and neither the location of the carboxylic acid moiety nor the stereochemistry of the C-terminal residue significantly affected the δ-receptor affinity of these derivatives. Extension of TIPP with an additional residue did not increase μ-receptor affinity, even though the position of the acidic group, which imparts δ-receptor selectivity to TIPP, was shifted relative to the carboxylic acid moiety of TIPP. The δ-receptor affinities of the TIP-d/l-Asx/Glx derivatives were found to be influenced mainly by the position of the carboxylic acid function rather than the stereochemistry of the C-terminal residue. TIP(P)-d/l-Lys(Ac)-OH derivatives exhibited moderate δ-receptor affinity (Kiδ = 16−28 nM). The most potent compounds found in the extended TIP(P) series were TIPP-d-Gln-OH and TIP-d-Gln-OH (Kiδ = 5 nM) which had similar affinities to TIPP.