In vitro proteolytic cleavage of the Escherichia coli Ada protein by the ompT gene product
Open Access
- 31 March 1989
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 171 (4) , 2249-2251
- https://doi.org/10.1128/jb.171.4.2249-2251.1989
Abstract
Down regulation of the adaptive response to alkylation damage in Escherichia coli has been proposed to occur by proteolytic cleavage of the regulatory Ada protein. In this paper, it is shown that proteolysis of the Ada protein as observed in cell extracts is caused by the ompT gene product. This protease, however, was not involved in switching off the adaptive response in vivo.This publication has 30 references indexed in Scilit:
- REGULATION AND EXPRESSION OF THE ADAPTIVE RESPONSE TO ALKYLATING AGENTSAnnual Review of Biochemistry, 1988
- Proteolytic processing of the Ada protein that repairs DNA O6-methylguanine residues in E. coliMutation Research/DNA Repair Reports, 1987
- The intracellular signal for induction of resistance to alkylating agents in E. coliCell, 1986
- Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocelluloseGene Analysis Techniques, 1984
- Microbial Envelope Proteins Related to IronAnnual Review of Microbiology, 1982
- Escherichia coli K-12 mutants that lack major outer membrane protein aFEMS Microbiology Letters, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Modification of a ferric enterobactin receptor protein from the outer membrane of Escherichia coliBiochemical and Biophysical Research Communications, 1978
- Outer membrane protein 3B ofEscherichia coliK-12: Effects of growth temperature on the amount of the protein and further characterization on acrylamide gelsFEMS Microbiology Letters, 1977
- A new pathway for DNA repair in Escherichia coliNature, 1977