MHC class II, tumour necrosis factor α, and lymphotoxin α gene haplotype associations with serological subsets of systemic lupus erythematosus
- 1 April 2006
- journal article
- other
- Published by Elsevier in Annals of the Rheumatic Diseases
- Vol. 65 (4) , 488-494
- https://doi.org/10.1136/ard.2005.039842
Abstract
Objective: To conduct a case–control study to investigate whether there are independent tumour necrosis factor α (TNFα) or lymphotoxin α (LTα) haplotype associations with SLE or with any of the major serological subsets of SLE. Methods: 157 patients with SLE were genotyped for HLA-DRB1, HLA-DQB1, TNFα, and LTα alleles by polymerase chain reaction and compared with 245 normal white controls. For TNFα, six single nucleotide polymorphisms (SNPs) at positions −1031, −863, −857, −308, −238, and +488 and for LTα three SNPs at positions +720, +365, and +249 were studied to assign six TNFα haplotypes (TNF1-6) and four LTα haplotypes (LTA1-4). All SLE patients had full serological profiles on serial samples. Results: The most significant association with SLE overall was with HLA-DR3 (pConclusions: The strongest association in this predominantly white population with SLE was between HLA-DR3 and anti-La, which seemed to account for any associations with TNFα alleles on an extended DR3 haplotype.Keywords
This publication has 52 references indexed in Scilit:
- HLA class II alleles associations of anticardiolipin and anti b2GPI antibodies in a large series of European patients with systemic lupus erythematosusLupus, 2000
- Polymerase chain reaction haplotyping using 3′ mismatches in the forward and reverse primers: application to the biallelic polymorphisms of tumor necrosis factor and lymphotoxin αTissue Antigens, 1997
- Distributions of HLA Class II Alleles in Autoantibody Subsets Among Norwegian Patients with Systemic Lupus ErythematosusScandinavian Journal of Immunology, 1995
- HLA‐DQB1 and ‐DQA1 typing by PCR amplification with sequence‐specific primers (PCR‐SSP) in 2 hoursTissue Antigens, 1993
- Major histocompatibility complex haplotypes and complement C4 alleles in systemic lupus erythematosus. Results of a multicenter study.Journal of Clinical Investigation, 1992
- HLA‐DR typing by PCR amplification with sequence‐specific primers (PCR‐SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor‐recipient matching in cadaveric transplantationTissue Antigens, 1992
- Identification of the HLA-DRB1∗04, -DRB1∗07, and -DRB1∗09 Alleles by PCR Amplification with sequence-specific primers (PCR-SSP) in 2 hoursHuman Immunology, 1992
- HLA-DQ gene complementation and other histocompatibility relationships in man with the anti-Ro/SSA autoantibody response of systemic lupus erythematosus.Journal of Clinical Investigation, 1990
- Chronic therapy with recombinant tumor necrosis factor-α in autoimmune NZB/NZW F1 miceClinical Immunology and Immunopathology, 1989
- HLA-DR antigens in systemic lupus erythematosus: association with specificity of autoantibody responses to nuclear antigens.Annals of the Rheumatic Diseases, 1987