High Performance Liquid Chromatographic Evaluation of a Low-Temperature Glassy Carbon Stationary Phase

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Abstract
A low temperature glassy carbon (LTGC) stationary phase coated on porous glassy carbon and zirconium oxide is chromatographically evaluated and compared to an uncoated porous glassy carbon stationary phase. The curing temperature of the LTGC was chosen to produce a surface having a reversed phase type retention mechanism. The LTGC coating of the porous glassy carbon reduced retention due to dispersive interactions while maintaining a similar retention mechanism as the untreated porous glassy carbon phase. The LTGC-coated zirconia produced an efficient column with classical reversed-phase retention behavior.