[The chromophore of blue dextran: a powerful ligand for the purification of interferon by affinity chromatography].
- 27 September 1976
- journal article
- Vol. 283 (6) , 709-11
Abstract
The chromophore of blue dextran can be used as ligand for affinity chromatography of interferon. One passage of a crude interferon preparation on a blue-dextran-sepharose column eliminates over 90% of the contaminating proteins. Desorption with an aqueous buffer of high molarity yields interferon preparations with specific activities of up to 4.5 X 10(6) units per milligram of protein and the recovery is 100%.Keywords
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