The implication of post‐mortem changes such as cell swelling, imbibition and autolysis on the elemental composition of body organs has been studied in rats. Liver has been chosen as an example. Retaining the liver inside the intact dead body for different periods of time at ambient temperature induced significant changes in its weight due to post‐mortem tissue degeneration. Livers from animals that were frozen at −15°C also showed significant decrease in weight when they were thawed on the third day. The effect of these changes on the concentrations of various elements depended on the association of the elements with extracellular fluid and intracellular components. For example, concentration of K+ was affected more by the lysis of the cell and sustained losses up to 30 per cent, in relation to the control values, while the total content was reduced by more than 40 per cent. as a result of both lysis and tissue liquefaction. For Na+, differences ranging from +10 to −20 per cent. in concentrations and +20 to −40 per cent. in total content were observed which could be explained by the movement of fluid in and out of the organ. Among trace elements, variations observed for iron ranged from −20 to +40 per cent., while both copper and zinc were found to fluctuate between −20 and +20 per cent. The loss observed in the total content in the liver for the five trace elements studied (Cu, Fe, Mn, Rb and Zn) was found to be about 20 to 40 per cent.