Angiogenesis in an in vivo model of adipose tissue development

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Abstract
SPECIFIC AIMSLittle is known about angiogenesis in adipose tissue, primarily because of the absence of reliable model systems. The general aim of these studies was to determine whether fat pads that develop when cultured 3T3-F442A cells are injected under the skin of nude mice form a suitable in vivo model system to study neovascularization of developing adipose tissue. One specific aim was to provide initial information about the morphology, kinetics of neovascularization, and gene expression profiles of the developing fat pads; another was to determine whether their new vasculature originated from bone marrow-derived endothelial progenitor cells or from preexisting host vessels.PRINCIPAL FINDINGS1. Fat pads formed by 3T3-F442A cells in nude mice are highly vascularized and morphologically similar to inguinal fatUsed to study adipogenesis, the 3T3-F442A in vivo fat pad model has not been used to examine adipose tissue neovascularization. In this model, 3T3-F442A preadipocytes are implanted subcutaneously...
Funding Information
  • National Institutes of Health (HL59549)
  • American Heart Association