A simple technique for accelerating the growth of the kidney disease bacterium Renibacterium salmoninarum on a commonly used culture medium (KDM2)

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Abstract
A simple technique for significantly accelerating the growth of Renibacterium salmoninarum (Rs), causative agent of bacterial kidney disease in salmonids, is described and evaluated. The technique is based on the well-known phenomenon, referred to as ''satellitism'' or ''cross-feeding'', in which a fastidious organism is induced to grow by placing it on an agar culture medium next to a non-fastidious feeder or ''nurse'' organism. In the present procedure, the nurse organism is actually a stock culture of Rs, a heavy suspension of which is drop-inoculated (25 .mu.l aliquot) onto the centre of a KDM2 culture plate. Samples suspected of containing Rs are peripherally drop-inoculated on the plate. Growth of the nurse culture occurs rapidly and modifies conditions in the culture plate such that growth of Rs cells in the peripheral samples is greatly accelerated. The effect is most pronounced when the number of Rs cells in peripheral samples is low. The mechanism underlying growth acceleration was not investigated.

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