Active center differences between cathepsins L and B: The S1 binding region
- 8 February 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 228 (1) , 128-130
- https://doi.org/10.1016/0014-5793(88)80600-8
Abstract
The substrate peptide bond cleaved by cathepsins B and L is determined not by the amino acid contributing the carboxyl group to this bond as in the case of serine proteases but rather by the presence of a neighboring amino acid with a large hydrophobic side chain. From a study of the inhibitory potency in a series, Cbz-Phe-X-CHN2, in which Phe promotes binding at S2 (terminology of [(1968) Biochem. Biophys. Res. Commun. 32, 898–902]) while the amino acid X probes S1, it is shown that this region of cathepsin L also has the ability to accommodate large hydrophobic side chains. In this respect cathepsin L differs from cathepsin B. Thus Cbz-Phe-Tyr(O-t-Bu)CHN2 inactivates cathepsin L with a rate 2.5 × 104 greater than that for cathepsin B.Keywords
This publication has 22 references indexed in Scilit:
- Autodegradation of lysosomal cysteine proteinasesBiochemical and Biophysical Research Communications, 1987
- The thiol proteinase inhibitors, Z-Phe-PheCHN2 and Z-Phe-AlaCHN2, inhibit lysosomal protein degradation in isolated rat hepatocytesBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- Paradoxical effect of leupeptin in vivo on Cathepsin B activityBiochemical and Biophysical Research Communications, 1983
- Rapid inactivation of cathepsin L by Z-Phe-PheCHN2 and Z-Phe-AlaCHN2Biochemical and Biophysical Research Communications, 1981
- Action of cathepsin L on the oxidized B‐chain of bovine insulinFEBS Letters, 1980
- A comparison of the behavior of chymotrypsin and cathepsin B towards peptidyl diazomethyl ketonesBiochemical and Biophysical Research Communications, 1979
- Inhibition of proteolytic activity of calcium activated neutral protease by leupeptin and antipainBiochemical and Biophysical Research Communications, 1978
- Isolation and characterization of E-64, a new thiol protease inhibitor.Agricultural and Biological Chemistry, 1978
- Cathepsin LEuropean Journal of Biochemistry, 1977
- On the active site of proteases. III. Mapping the active site of papain; specific peptide inhibitors of papainBiochemical and Biophysical Research Communications, 1968