Preparation Of Phage-Free Pyocin Extracts For Use In The Typing Of Pseudomonas Aeruginosa

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Abstract
A simple method is described for the inactivation of contaminating bacteriophage in concentrated pyocin preparations prepared from induced lysates of Pseudomonas aeruginosa. It is possible to destroy the plaque-forming ability of the bacteriophage by treatment with ultraviolet light and at the same time to preserve approximately 65 per cent. of the pyocin activity.

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