A monoclonal antibody detecting the Ly-9.2 (Lgp 100) cell-membrane alloantigen
- 1 December 1980
- journal article
- research article
- Published by Springer Nature in Immunogenetics
- Vol. 11-11 (1) , 65-74
- https://doi.org/10.1007/bf01567770
Abstract
A mouse monoclonal cell line (20-1.5) was produced by the cell fusion method and the antibody secreted by this line defined the Ly-9.2 specificity — the reciprocal specificity to that previously identified as the Lgp 100 or the T100 molecule. Although most concentrated on lymph-node cells, the antigen is also found on thymocytes, spleen and bone-marrow cells as well as liver and brain tissue. The monoclonal antibody precipitates a 100000 molecular weight moiety from thymocytes. The antigenic specificities appear to be highly immunogeneic and antibodies to these specificities contaminate many antisera. These sera are noncytotoxic as is the case with the monoclonal antibody even though it is of the IgG2a subclass. As with T100 or Lgp 100, theLy-9 locus appears to be linked to theH-25 locus.Keywords
This publication has 16 references indexed in Scilit:
- A new mouse lymphoid alloantigen (Lgp100) recognized by a monoclonal rat antibodyImmunogenetics, 1979
- Murine Lymphocyte Surface AntigensPublished by Elsevier ,1979
- The firstH- 2 mutant workshopImmunogenetics, 1978
- Comparison of antigens recognized by xenogeneic and allogeneic anti-Ia antibodies: Evidence for two classes of Ia antigensImmunogenetics, 1978
- DETECTION OF MOUSE ALLOANTIBODIES BY ROSETTING WITH PROTEIN A-COATED SHEEP RED BLOOD CELLSTransplantation, 1978
- A sensitive rosetting method for detecting subpopulations of lymphocytes which react with alloantiseraJournal of Immunological Methods, 1978
- A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cellsSomatic Cell and Molecular Genetics, 1977
- A procedure for removing red cells and dead cells from lymphoid cell suspensionsJournal of Immunological Methods, 1975
- A one‐step procedure for separating mouse T and B lymphocytesEuropean Journal of Immunology, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970