BENZO[ALPHA]PYRENE METABOLIZING ACTIVITY OF CULTURED-CELLS AS DETERMINED BY A SIMPLIFIED RADIOMETRIC METHOD

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 68  (6) , 737-744
Abstract
Benzo[a]pyrene metabolism in various kinds of mammalian cultured cells was measured by a simplified method. Cells growing in the bottom of glass test tubes were treated with 2 .mu.M of 14C-benzo[a]pyrene for 24 h. Unmetabolized benzo[a]pyrene was extracted with organic solvents in the same test tube, and the amount of water-soluble products recovered in the aqueous phase was measured by a liquid scintillation counter. Among rodent embryo cells, embryo cells of C3H/He mice and Syrian hamsters showed a higher activity in benzo[a]pyrene metabolism than those of DDD, AKR and C57BL/6 mice. Several embryo cell lines sensitive to density-dependent inhibition, such as Y-AK, DL1, and C3H/10T1/2, actively metabolized benzo[a]pyrene to water-soluble products. The metabolizing activity of C3H/He mouse embryo cells was enhanced by pretreatment with benz[a]anthracene. The amount of water-soluble products formed by phytohemagglutinin-stimulated human lymphocytes was less than that formed by most cultured cell lines and embryo cells, but clearly increased with extension of culture days.

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