A Modified Air- drying Method for a Chromosome Study of a Solid Tumor

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Abstract
[In Jap. with Engl. Summ.][long dash]An air-drying technique was tried to facilitate the analysis of chromosome complements in cells of a solid tumor as follows: thoroughly wash the material in balanced salt solution, and mince on a watch glass as well as possible; suspend in hypotonic solution in a mixture of Hanks solution and distilled water, 1:3, for 20 min; centrifuge at 200 rpm for 2 min, and take off the coarse precipitate; centrifuge at 800 rpm for 5 min, and discard almost supernatant, leaving a small volume of supernatant in a centrifuge bottle; resuspend cells gently; put a centrifuge bottle in ice water, then add cold Carnoy''s fluid very slowly; leave for 30 min in ice water; centrifuge at 800 rpm for 10 min; decant and suspend in about 0. 5 to 1 ml of ice-cold Carnoy''s fluid; drop on to wet slide (Keeping in 50% cold alcohol) from a fine pipette; the drops should be very small; spreading may be accelerated by blowing; dry quickly over a spirit flame; stain with 0.75% dahlia in 30% acetic acid for 1 to 2 min. The method described above achieved a fairly uniform and complete flattening figures of metaphase in cells of a solid tumor, which were difficult to observe by manual squashing method.

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