ELECTRON MICROSCOPY OF SALIVARY GLAND VIRUSES

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Abstract
A human and a mouse strain of salivary gland virus were examined. The human strain was transmitted, prior to examination, to tissue cultures derived from human myometrial cells, while the mouse strain was examined in mice inoculated intraperitoneally. The nuclear forms associated with both strains were morphologically similar. Nuclear inclusions, com posed of particles interspersed with dense clumped chromatin, were a striking feature of infected cells. The cytoplasmic forms were of 2 types- one a 300 to 500 mu homogeneous dense spherical form, and the other a target-like form composed of a central dense dot in a pale zone surrounded by a dense shell the entire configuration measuring 100 to 180 mu The target-like particle appeared identical in both strains. The spherical cytoplasmic forms in cells infected with human strain appeared to be solid, while in cells infected with mouse strain there was evidence of formation of target-like forms within the spheres. Possible mechanisms by which infection of the cell may occur, as well as possible mechanisms and sites of multiplication of virus, are discussed.