HUMAN PROSTATIC ADENOMA AND CARCINOMA - TRANSPLANTATION OF CULTURED-CELLS AND PRIMARY TISSUE FRAGMENTS IN NUDE MICE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 13  (6) , 395-403
Abstract
Tissues from human prostatic carcinomas and adenomas and cells from permanent cell lines originating from such tumors were transplanted into the subcutaneous space of nude mice. A total of 208 such transplantations were done. For transplantation of primary tumor tissue several techniques were used. Transplantation of single pieces of about 50 mg of tissue from carcinomas and adenomas provided the best results. With this technique carcinoma tissue was recovered from 5 of 10 tumors transplanted into 46 nude mice. Carcinoma was found in 7 of 25 nude mice that were castrated and treated with testosterone but only once in 21 transplantations into castrates without testosterone substitution. This may suggest that heterotransplants of human prostatic carcinoma tissue can be established more easily in testosteronized animals. In the case of adenoma transplants the histologic picture was strikingly similar to the original tumors. Secretory activity was found in some acini, squamous metaplasia in others. Heterotransplantation of cells from permanent cell lines resulted in fast growing carcinomas without similarity to the original tumors. Dependence on hormones for growth of EB 33 and MA 160 cells in nude mice was not shown.

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