Synthesis and Hybridization Characteristics of Oligodeoxynucleotide-Alkaline Phosphatase Conjugates

1 January 1987

journal article
research article
Published by Taylor & Francis in Nucleosides and Nucleotides

Vol. 6 (1-2) , 541-542
https://doi.org/10.1080/07328318708056281

Abstract

Modified oligodeoxynucleotides, 20 to 30 bases in length and containing “linker arm” bases, are chemically synthesized, then attached covalently to alkaline phosphatase in a 1:1 conjugate through a 19 atom spacer. Such conjugates hybridize rapidly and selectively to complementary sequences similar to unmodified oligomers, and allow colorimetrlc and fluorogenic detection of hybrids.

Keywords

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