T-CELL SUB-POPULATIONS IDENTIFIED BY MONOCLONAL-ANTIBODIES AFTER HUMAN MARROW TRANSPLANTATION .1. HELPER INDUCER AND CYTOTOXIC-SUPPRESSOR SUBSETS

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 59  (6) , 1292-1298
Abstract
Peripheral blood helper-inducer and cytotoxic-suppressor T-cell subpopulations in patients receiving marrow transplants for the treatment of acute leukemia or severe aplastic anemia were quantitated on the fluorescence-activated cell sorter (FACS) using the monoclonal antibodies OKT4 and OKT8, respectively. The relative (percent) and absolute number of OKT4+ cells were severely and persistently depleted for up to 2.7 yr posttransplant. The percent and absolute number of OKT8+ cells began to recover within the first 60 days of transplant and subsequently remained at normal or high levels for periods of up to 7.3 yr. There was no significant differences in percent or absolute numbers of OKT8+ cells for patients with or without acute graft-vs.-host disease (GVHD). The reversal of the normal OKT45:OKT8 ratio (2:1) occurred regardless of whether the recipient was given an allogeneic, syngeneic or autologous transplant and regardless of whether or not acute or chronic GVHD developed. The reversed ratio was due in the first 3 mo. posttransplant to low numbers of OKT4+ cells and later to a combination of low numbers of OKT4+ and high numbers of OKT8+ cells. Normalization and then an increase in the number of OKIT8+ cells correlated with increasing time posttransplant and not with resolution of acute GVHD.

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