STUDY OF AXIS-SHIELD NEW %CDT IMMUNOASSAY FOR QUANTIFICATION OF CARBOHYDRATE-DEFICIENT TRANSFERRIN (CDT) IN SERUM

Open Access
Publisher Website
Google Scholar
Abstract
— Carbohydrate-deficient transferrin (CDT) in serum has emerged as a useful biochemical marker for identifying current alcohol misuse and monitoring abstinence. This study evaluated the performance of Axis–Shield new %CDT turbidimetric immunoassay (TIA; microtitre and Cobas Mira applications). Comparison was made with the previous Axis %CDT-TIA immunoassay (reference value n = 100) and this was not significantly different from healthy non-drinkers (1.88 ± 0.43%, 1.3–2.9%, n = 14), whereas abstinent alcohol patients showed slightly higher values (2.26 ± 0.41, 1.7–3.4, n = 25). In chronic heavy drinkers (mean daily intake 225 ± 137 g ethanol according to self-report), the %CDT values were markedly increased (6.33 ± 4.01%, 1.2–18.0%, n = 107). There was no significant difference in %CDT values between male and female social drinkers. The reference value of the new %CDT assay to be used in clinical practice was tentatively set at r = 0.986, P < 0.0001) and %CDT-HPLC (r = 0.978, P < 0.0001). The specificity of the %CDT assay in healthy social drinkers was 98% (%CDT-TIA 100%, %CDT-HPLC 99%) and the sensitivity for any drinking during last week in the alcohol patients was 75% (%CDT-TIA 71%, %CDT-HPLC 80%). The new Axis–Shield %CDT assay can be recommended for routine use. However, whenever a positive immunoassay test result could lead to serious consequences for the individual, it is recommended to confirm the CDT result by the HPLC technique.

This publication has 0 references indexed in Scilit: