Thrombin Interactions with Central Nervous System Tissue and Implications of These Interactionsa
- 16 December 1986
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 485 (1) , 314-322
- https://doi.org/10.1111/j.1749-6632.1986.tb34593.x
Abstract
A monospecific antibody was developed to human alpha-thrombin. This antibody stained neurons but not astrocytes in murine spinal cord cultures incubated with 1-10 nM alpha-thrombin using the avidin-biotin-peroxidase technique. Staining did not occur when the primary or linking antibodies were eliminated, and staining was blocked with hirudin. Preliminary studies showed release of arachidonic acid from the cultures when exposed to thrombin. It was proposed that arachidonate release from the membranes of neurons upon exposure to thrombin was similar to that observed in platelets, for example, by activation of phospholipases. Moreover, prostanoids were formed that could have a deleterious effect on cellular elements in the central nervous system. The potential role of thrombin receptors on neurons was discussed.Keywords
This publication has 13 references indexed in Scilit:
- Neurotransmitter receptors mediate cyclic GMP formation by involvement of arachidonic acid and lipoxygenase.Proceedings of the National Academy of Sciences, 1984
- Thrombin Stimulation of Guanosine 3′,5′-Monophosphate Formation in Murine Neuroblastoma Cells (Clone N1E-115)Science, 1983
- Phospholipase activities of rat brain cytosol occurrence of phospholipase c activity with phosphatidylcholineBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- Interaction of Brain Synaptic Vesicles Induced by Endogenous Ca 2+ -Dependent Phospholipase A 2Science, 1982
- Eicosanoids: Prostaglandins, Thromboxanes, Leukotrienes, and Other Derivatives of Carbon‐20 Unsaturated Fatty AcidsJournal of Neurochemistry, 1982
- THROMBIN SPECIFICITY*Annals of the New York Academy of Sciences, 1981
- Enzymic regulation of arachidonate metabolism in brain membrane phosphoglyceridesLipids, 1979
- Direct linkage of thrombin to its cell surface receptors in different cell typesJournal of Supramolecular Structure, 1979
- SIGNIFICANCE OF PHOSPHOLIPASE A FOR PROSTAGLANDIN FORMATIONAnnals of the New York Academy of Sciences, 1971
- The isolation of IgG from mammalian sera with the aid of caprylic acidArchives of Biochemistry and Biophysics, 1969