FACTORS AFFECTING THE MEASUREMENT OF PROTEOLYTIC ACTIVITY OF THYROID TISSUE
- 1 April 1950
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 46 (4) , 353-358
- https://doi.org/10.1210/endo-46-4-353
Abstract
Thyroid glands of adult dogs contain a factor in the presence of which tyrosine is formed from a hemoglobin substrate. The activity of this factor is destroyed by heat. Tyrosine was estimated by the colorimetric method using the Folin-Ciocalteu phenol reagent for development of color; tissue, reagent, and substrate "blanks" were detd. and are very small. The maximum proteolytic activity of thyroid tissue occurs at a pH of about 3.55 produced by acetic acid.Keywords
This publication has 2 references indexed in Scilit:
- THE PROTEOLYTIC ACTIVITY OF NORMAL AND PATHOLOGICAL HUMAN THYROID TISSUE1Journal of Clinical Endocrinology & Metabolism, 1946
- THE ESTIMATION OF PEPSIN, TRYPSIN, PAPAIN, AND CATHEPSIN WITH HEMOGLOBINThe Journal of general physiology, 1938