Inactivation of beef heart mitochondrial F1‐ATPase by the 2′,3′‐dialdehyde derivatives of adenine nucleotides
- 9 April 1984
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 169 (1) , 101-106
- https://doi.org/10.1016/0014-5793(84)80297-5
Abstract
Beef heart mitochondrial F1-ATPase was inactivated by the 2′,3′-dialdehyde derivatives of ATP, ADP and AMP (oATP, oADP, oAMP). In the absence of Mg2+, inactivation resulted from the binding of 1 mol nucleotide analog per active unit of F1. The most efficient analog was oADP, followed by oAMP and oATP. Complete inactivation was correlated with the binding of about 11 mol [14C]oADP/mol F1. After correction for non-specific labeling, the number of specifically bound [14C]oADP was 2–3 mol per mol F1. By SDS-polyacrylamide gel electrophoresis, [14C]oADP was found to bind covalently mainly to the α and β subunits. In the presence of Mg2+, oATP behaved as a substrate and was slowly hydrolyzed.Keywords
This publication has 29 references indexed in Scilit:
- Interactions between the mitochondrial adenosinetriphosphatase and periodate-oxidized adenosine 5'-triphosphate, an affinity label for adenosine 5'-triphosphate binding sitesBiochemistry, 1982
- Affinity labeling of rabbit muscle pyruvate kinase with dialdehyde-ADPBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- A difference in the affinity labeling of Ca2+, Mg2+-activated ATPases of normal and unc A strains of Escherichiacoli by the 2′,3′-dialdehyde derivative of adenosine 5′-diphosphateBiochemical and Biophysical Research Communications, 1980
- Affinity labeling of catalytic and regulatory sites of pig heart mitochondrial F1-ATPase by 5'-p-fluorosulfonylbenzoyladenosineBiochemistry, 1979
- Reactivity of mitochondrial F1-ATPase to dicyclohexylcarbodiimide. Inactivation and binding studiesBiochemistry, 1979
- Interaction of azidonitrophenylaminobutyryl—ADP, a photoaffinity ADP analog, with mitochondrial adenosine triphosphatase. Identification of the labeled subunitsFEBS Letters, 1977
- Specific photolabelling of beef-heart mitochondrial ATPase by 8-azido-ATPBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977
- Arylazido aminopropionyl ATP, an active site directed photoaffinity reagent for mitochondrial adenosine triphosphataseBiochemical and Biophysical Research Communications, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Pyruvate Carboxylase Affinity Labelling of the Magnesium Adenosine Triphosphate Binding SiteEuropean Journal of Biochemistry, 1976