MODIFICATIONS IN BIPHASIC LIQUID-SCINTILLATION VIAL SYSTEM FOR RADIOMETRY

  • 1 January 1980
    • journal article
    • research article
    • Vol. 21  (5) , 480-483
Abstract
Several modifications of the biphasic liquid-scintillation vial system for radiometry [used for detecting microbial activity] were tried to improve the counting efficiency. The biphasic system consisted of an inner sterile vial containing medium and substrate, and an outer liquid-scintillation vial lined on the inside with filter paper impregnated with scintillation fluors and alkali. The system gave an overall counting efficiency of 14.6%. Substitution of methanolic NaOH for impregnation of the paper raised the counting efficiency to 29.1%. Further enhancement to 33.8% was possible by lining only half of the outer vial with filter paper, allowing improved optical transmission of scintillation light. Increasing the amount of fluor did not change the efficiency significantly. A complete interchange in the system, with half of the inner vial lined with filter paper and the outer vial containing the medium and substrate, gave the highest efficiency (36.9%). This allowed the use of larger amounts of medium and the inoculum.

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