Immunohistochemical Identification of Tissue Culture Cells

Publisher Website
Google Scholar
Abstract
Summary Cell lines of different species have been shown to be differentiated by immunohistochemical technics using properly absorbed antisera. In the work reported here the antisera were prepared against human and mouse tissues and reacted strongly with the connective tissue of the corresponding species in sections of adult human or adult mouse organs, respectively. These antisera also reacted individually with tissue culture cells of human and mouse organs. In all cases the use of the proper tissue in sufficient amounts to absorb the antiserums plays a crucial part in being able to demonstrate the specific staining of cells. Differentiation can be made even within the cell lines of a single species when unique components are present in one of them. The use of anti-myosin serum to detect muscle cells is an example. The technic of using paired labeled antiserum quickly and effectively demonstrates that very slight contamination can be recognized. Such a small contamination would be difficult to detect by other tests such as chromosome morphology technics where the cell must be in the metaphase before it can be observed and chromosomes of each cell counted.

This publication has 1 reference indexed in Scilit: