SCREENING SOYBEAN FOR RESISTANCE TO HETERODERA-GLYCINES ICHINOHE USING MONOXENIC CULTURES

  • 1 January 1982
    • journal article
    • research article
    • Vol. 14  (4) , 593-594
Abstract
A simple, rapid and inexpensive method for evaluation of host-parasite interactions, based on monoxenic cultures, is described. Axenic root explants of G. max (L.) Merr., cultured on a holidic agar medium, were inoculated with axenic 2nd-stage larvae of H. glycines Ichinohe, Race 3. A clear separation of susceptible and resistant cultivars, based on numbers of mature female nematodes present after 3 wk at 25.degree. C, was observed. This method should aid researchers in the evaluation of the host response to infection by H. glycines.

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