UNUSUAL BEHAVIOUR OF A PROTEIN: REVERSIBLE QUANTITATIVE PRECIPITATION OF STAPHYLOCOCCAL DELTA-HAEMOLYSIN BY LOW CONCENTRATIONS OF SOME ORGANIC SOLVENTS
- 12 January 2009
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 8 (3) , 233-236
- https://doi.org/10.1111/j.1399-3011.1976.tb02499.x
Abstract
Staphylococcal .delta.-hemolysin is precipitated from watery solutions by low concentrations of chloroform. The effect on this precipitation of pH, kind and concentration of salts, and concentration of, and time of exposure to, chloroform were investigated. Some but not all of the other solvents tested cause a similar precipitation. The hemolytic activity is not destroyed and under appropriate conditions precipitation is quantitative. The dry lysin will take up nearly double its weight of chloroform when exposed to the vapor. Some is bound strongly.This publication has 2 references indexed in Scilit:
- A New Method for the Preparation and Some Properties of Staphylococcal Delta-haemolysinJournal of General Microbiology, 1971
- Staphyococcal δ-hemolysin I. Purification and chemical propertiesBiochimica et Biophysica Acta, 1963