Purification of Twelve Cyanogen Bromide Fragments from Bovine Plasma Fibronectin and the Amino Acid Sequence of Eight of Them

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Abstract
Twelve cyanogen bromide fragments (CB1.sbd.12) from bovine plasma fibronectin were isolated and 8 of these completely sequenced. Altogether they account for 502 of the total expected 1880 residues in each of the 2 chains of fibronectin. Four of these fragments (CB1.sbd.4) constitute residues 1.sbd.289 in fibronectin with CB4 overlappping the N-terminal 29-kDa [kilodalton] plasmic fragment to the second plasmic fragment, of 170-kDa in fibronectin. Fragments CB 5.sbd.9 are all contained within a 45-kDa gelatin-binding region, which is N-terminal in the 170-kDa fragment. The sequence of 2 of these 5 fragments in the 45-kDa fragment (CB7-8) contains 2 mutually homologous stretches with 57% sequence identity. Another 2 fragments (CB10-11) are derived from the heparin-binding region of the 170-kDa fragment. CB12 constitutes the C-terminal 13-residue stretch in fibronectin and contains a partly phosphorylated serine residue in the C-terminal sequence: -Arg-Glu-Asp-Ser(P)-Arg-Glu.

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