Somatic Embryogenesis and Plant Regeneration from Immature Embryos of Strawberry
Open Access
- 1 February 1984
- journal article
- Published by American Society for Horticultural Science in HortScience
- Vol. 19 (1) , 71-72
- https://doi.org/10.21273/hortsci.19.1.71
Abstract
Immature zygotic embryos of strawberry (Fragaria × ananassa Duch.), cultured on Murashige and Skoog (MS) basal medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylamino purine (BA), developed morphogenically active structures formed by folding of the cotyledonary tissue on their surfaces; these structures eventually developed into somatic embryos. The frequency for initiating embryogenic tissue was highest in the presence of 5 mg/liter 2,4-D, 0.5 mg/liter BA, arid 500 mg/liter casein hydrolysate. Few of the embryogenic tissues developed into somatic embryos. Morphologically normal plants were obtained from somatic embryos that were transferred to MS medium supplemented with 1 mg/liter gibberellic acid (GA3) or 0.5 mg/liter BA + 0.1 mg/liter naphthaleneacetic acid (NAA).Keywords
This publication has 0 references indexed in Scilit: