Murine Cytomegalovirus Major Immediate-Early Enhancer Region Operating as a Genetic Switch in Bidirectional Gene Pair Transcription
- 15 July 2007
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 81 (14) , 7805-7810
- https://doi.org/10.1128/jvi.02388-06
Abstract
Enhancers are defined as DNA elements that increase transcription when placed in any orientation relative to a promoter. The major immediate-early (MIE) enhancer region of murine cytomegalovirus is flanked by transcription units ie1/3 and ie2 , which are transcribed in opposite directions. We have addressed the fundamental mechanistic question of whether the enhancer synchronizes transcription of the bidirectional gene pair (synchronizer model) or whether it operates as a genetic switch, enhancing transcription of either gene in a stochastic alternation (switch model). Clonal analysis of cytokine-triggered, transcription factor-mediated MIE gene expression from latent viral genomes provided evidence in support of the switch model.Keywords
This publication has 32 references indexed in Scilit:
- CD8 T Cells Control Cytomegalovirus Latency by Epitope-Specific Sensing of Transcriptional ReactivationJournal of Virology, 2006
- Autorepression of the Human Cytomegalovirus Major Immediate-Early Promoter/Enhancer at Late Times of Infection Is Mediated by the Recruitment of Chromatin Remodeling Enzymes by IE86Journal of Virology, 2006
- The IE2 promoter/enhancer region from mouse CMV provides high levels of therapeutic protein expression in mammalian cellsBiotechnology & Bioengineering, 2006
- An Abundance of Bidirectional Promoters in the Human GenomeGenome Research, 2004
- The Human Cytomegalovirus Major Immediate-Early Enhancer Determines the Efficiency of Immediate-Early Gene Transcription and Viral Replication in Permissive Cells at Low Multiplicity of InfectionJournal of Virology, 2003
- An Essential Role of the Enhancer for Murine Cytomegalovirus In Vivo Growth and PathogenesisJournal of Virology, 2003
- Bidirectional Gene OrganizationCell, 2002
- Requirement of Multiple cis -Acting Elements in the Human Cytomegalovirus Major Immediate-Early Distal Enhancer for Viral Gene Expression and ReplicationJournal of Virology, 2002
- Reactivation of the Human Cytomegalovirus Major Immediate-Early Regulatory Region and Viral Replication in Embryonal NTera2 Cells: Role of Trichostatin A, Retinoic Acid, and Deletion of the 21-Base-Pair Repeats and ModulatorJournal of Virology, 2001
- A Survey of Exact Inference for Contingency TablesStatistical Science, 1992