Dopamine receptor binding sites in the rat superior colliculus

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Abstract
Following intravenous administration of [3H]spiperone or [3H]N,n‐propylnorapomorphine (NPA) to rats, radioactivity derived from the ligands accumulated in the striatum and superior colliculus when compared with cerebellar levels. The accumulation of [3H]spiperone in both areas was prevented by intraperitoneal administration of (+)‐butaclamol, haloperidol and sulpiride but not by (‐)‐butaclamol, cinanserin, propranolol or prazosin. The accumulation of [3H]NPA was prevented by administration of (+)‐butaclamol, haloperidol and apomorphine but not by (‐)‐butaclamol. In in‐vitro experiments, membrane preparations from the superior colliculus showed a small number of specific binding sites for both [3H]spiperone and [3H]NPA. The dissociation constant (KD) for [3H]NPA was not different from that for striatal preparations but that for [3H]spiperone was 10−fold higher. We conclude that dopamine receptors may be present within the superior colliculus.