GM-CFC growth in chronic granulocytic leukaemia is not affected by a soluble inhibitor released by aplastic anaemia T-cells or mitogen-primed normal T-lymphocytes

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Abstract
Peripheral blood (PB) and bone marrow (BM) cells were obtained from 12 patients with chronic granulocytic leukemia (CGL) and cultured in agar for granulocyte macrophage colony formation (GM-CFC). PB and BM CGL cells were also co-cultured with T-lymphocytes from patients with immune severe aplastic anemia (SAA), or with T-cells from healthy donors primed with pokeweed mitogen (PWM). The supernatants of SAA and PWM primed T-cells were also added to CGL cells grown in agar. Normal marrow cells obtained from 8 healthy donors were used to set up control cultures and co-cultures. T-cells derived from SAA paients and their supernatants suppress GM-CFC growth of normal marrow cells but not of PB nor BM CGL cells. Normal T-cells primed wiht PWM and their supernatants suppress normal marrow GM-CFC but not colony formation of BM nor PB CGL cells. Mediators which are effective in regulating normal myeloid progenitor cells fail to inhibit the in vitro growth of GM-CFC from CGL patients.