Enrichment of Auxotrophic Mutants in Streptomyces griseus
- 1 September 1980
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 40 (3) , 675-677
- https://doi.org/10.1128/aem.40.3.675-677.1980
Abstract
A method for the isolation and enrichment of tryptophan auxotrophic mutants of the indolmycin-producing strain Streptomyces griseus ATCC 12648 was developed, using penicillin selection. With UV irradiation of 2.2 × 10 4 μW cm −2 , a mutation rate to tryptophan auxotrophy of 5.6 × 10 −4 was achieved. With 300 μg ml −1 of penicillin G, an enrichment of tryptophan auxotrophs of about 1,000-fold was attained. Approximately 40% of all survivors were tryptophan auxotrophs.This publication has 3 references indexed in Scilit:
- Indolmycin Inhibits Prokaryotic Tryptophanyl-tRNA LigaseEuropean Journal of Biochemistry, 1976
- Identity of streptomycete producing antibiotic PA155AJournal of Bacteriology, 1966