REGULATION OF ANTIBODY-RESPONSE IN DIFFERENT IMMUNOGLOBULIN CLASSES .2. INDUCTION OF INVITRO IGE ANTIBODY-RESPONSE IN MURINE SPLEEN-CELLS AND DEMONSTRATION OF A POSSIBLE INVOLVEMENT OF DISTINCT T-HELPER CELLS IN IGE AND IGG ANTIBODY-RESPONSES

  • 1 January 1977
    • journal article
    • research article
    • Vol. 118  (3) , 840-845
Abstract
In vitro induction of anti-DNP [dinitrophenol] Ig[immunoglobulin]E as well as IgG1 and IgG2a antibody responses was shown in murine spleen cell culture. Spleen cells primed 3 times with 1 .mu.g of DNP-OA [ovalbumin] or DNP-Asc [Ascaris suum extract] produced significant amounts of anti-DNP IgE as well as IgG antibodies by the in vitro stimulation with DNP-OA or DNP-Asc, respectively. Collaboration between DNP-primed B [bone marrow-derived] cells and carrier-primed T [thymus-derived] cells was required for the induction of IgE and IgG antibodies with DNP-coupled T cell-dependent antigen. Carrier-specific T cells induced with a low dose of Asc (0.01 .mu.g) showed helper function only on IgE antibody response, whereas T cells primed with a higher dose of Asc (10 .mu.g) cooperated only with IgG-B cells. T cells primed with Asc in CFA [complete Freund''s adjuvant] showed helper function mainly on IgG antibody response but not on IgE antibody response. The presence of a distinct population of T helper cells for IgE and IgG antibody responses was indicated. T-independent antigen (DNP-Ficoll) induced anti-DNP IgE and IgG antibody responses in DNP-primed spleen cell population without the requirement of the collaboration of helper T cells.