The effects of a disulphonic stilbene on chloride and bicarbonate transport in the turtle bladder.

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Abstract
In turtle bladders bathed by Na-free media containing Cl- and .**GRAPHIC**. the mucosa is electropositive to the serosa. Serosally applied 4-acetamido-4''-isothiocyanostilbene-2,2''-disulfonic acid (SITS) reduces the p.d. [potential difference] and Isc [short circuiting current] by 100%, and reduces the rate of net Cl- reabsorption in some bladders but not in others. In the absence of exogenous .**GRAPHIC**. (Cl- present), SITS reduces the p.d. and Isc by 100%, and reduces the rate of mucosal acidification by 80%. Net Cl- reabsorption is not detectable under these .**GRAPHIC**. bathing conditions. In the absence of exogenous Cl- .**GRAPHIC**. present), the mucosa is also electropositive to the serosa and serosally applied SITS reverses the orientation of the p.d. and Isc so that the mucosa becomes electronegative to the serosa. This constitutes sufficient evidence for the active, electrogenic secretion of an anion, probably .**GRAPHIC**. An analogue of discrete, electrogenic pumps and paths may exist for the reabsorption of Na+, Cl- and .**GRAPHIC**. and the secretion of .**GRAPHIC**.