On-line HPLC/GC Techniques for the Analyses of Biomass Derived Hydrocarbons

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Abstract
An automated on-line HPLC/GC technique was developed for the analysis of biomass extractables (“biocrude”) as renewable sources of energy. The method was developed with a simulated “biocrude” mixture of n-alkane and terpenoid) standards, and was applied to a sample from Pedilanthus macrocarpus (Euphorbiaceae). Hydrocarbon group-type separations were achieved using normal-phase HPLC. On-line injections into a GC equipped with a WCOT OV-101 glass capillary column provided separations of the individual constituents. The technique can be used to monitor the agronomic development of bioenergy crops in order to optimize the production of the economically significant components.

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