Germ Line Transmission of a Yeast Artificial Chromosome Spanning the Murine α 1 (I) Collagen Locus
- 26 March 1993
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 259 (5103) , 1904-1907
- https://doi.org/10.1126/science.8096090
Abstract
Molecular complementation of mutant phenotypes by transgenic technology is a potentially important tool for gene identification. A technology was developed that allows the transfer of a physically intact yeast artificial chromosome (YAC) into the germ line of the mouse. A purified 150-kilobase YAC encompassing the murine gene Col1a1 was efficiently introduced into embryonic stem (ES) cells via lipofection. Chimeric founder mice were derived from two transfected ES cell clones. These chimeras transmitted the full length transgene through the germ line, generating two transgenic mouse strains. Transgene expression was visualized as nascent transcripts in interphase nuclei and quantitated by ribonuclease protection analysis. Both assays indicated that the transgene was expressed at levels comparable to the endogenous collagen gene.Keywords
This publication has 32 references indexed in Scilit:
- Transgenic mice containing a human heavy chain immunoglobulin gene fragment cloned in a yeast artificial chromosomeNature Genetics, 1993
- Higher Level Organization of Individual Gene Transcription and RNA SplicingScience, 1993
- Targeted mutation of the DNA methyltransferase gene results in embryonic lethalityPublished by Elsevier ,1992
- Bicolor fluorescence in situ hybridization to intron and exon mRNA sequencesExperimental Cell Research, 1991
- Transfer of the human HPRT and GART genes from yeast to mammalian cells by microinjection of YAC DNASomatic Cell and Molecular Genetics, 1991
- Development and applications of a molecular genetic linkage map of the mouse genomeTrends in Genetics, 1991
- Human glucose-6-phosphate dehydrogenase gene carried on a yeast artificial chromosome encodes active enzyme in monkey cellsGenomics, 1990
- Complete cloning of the duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individualsCell, 1987
- Chromosomal mapping of four different integration sites of Moloney murine leukemia virus including the locus for α1(I) collagen in mouseCytogenetic and Genome Research, 1986
- Characterization of the human factor VIII geneNature, 1984