A SCANNING AND TRANSMISSION ELECTRON MICROSCOPIC EXAMINATION OF RICKETTSIA TSUTSUGAMUSHI-INFECTED HUMAN ENDOTHELIAL, MRC-5, AND L-929 CELLS
- 1 January 1985
- journal article
- Published by Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis in Japanese Journal of Medical Science and Biology
- Vol. 38 (3) , 125-139
- https://doi.org/10.7883/yoken1952.38.125
Abstract
Monolayers of primary human endothelial cells were infected with the Karp strain of Rickettsia tsutsugamushi and examined by scanning and transmission electron microscopy. The results were compared with those obtained with similarly infected L-929 and MRC-5 cells and with uninfected cells of all three types. The rickettsiae grew to slightly higher titers in the human endothelial cells. Transmission electron microscopy revealed significant changes in the host cell organelles; a reduction in ribosome-coated endoplasmic reticulum and in Golgi activity, swelling of mitochondria, and an increase in vacuolation within the cytoplasm. Since human endothelial cells are known to retain their in vivo structural and functional qualities when cultured in vitro, it is likely that these effects are similar to those which occur during the infectious process in human scrub typhus.Keywords
This publication has 1 reference indexed in Scilit:
- Fixation for Scanning Electron MicroscopyPublished by Elsevier ,1981