INFLUENCE OF OSMOTIC PRESSURE ON SPORULATION BY BACILLUS SUBTILIS

1 July 1938

journal article
research article
Published by Oxford University Press (OUP) in Plant Physiology

Vol. 13 (3) , 649-653
https://doi.org/10.1104/pp.13.3.649

Abstract

Osmotic pressures up to 23 atm. were variously induced in 0.5% Bacto-peptone water by additions of KC1, NaCl, MgSO4, MgCl2, CaCl2, Na acetate, glycerol, and lactose. The % of sporulating cells in each medium was detd. after 48 and after 96 hrs. of incubation. The percentages of sporulating cells in media with osmotic pressures of 2 to 18 atm. are not significantly different. There is slightly less sporulation in media with pressures above 18 atm. and below 2 atm. The specific influence effect of the chemicals used seems to exert a greater influence upon sporulation by Bac. subtilis than does osmotic pressure.

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