Respiratory Mast Cells and Basophiloid Cells

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Abstract
The content of 3′,5′-adenosine monophosphate (cAMP) in free bronchial respiratory cells (RC) from dogs and rhesus monkeys was determined and compared with that of human peripheral blood leukocytes. The effects of β-adrenergic stimulating agents and concanavalin A on RC obtained from the bronchi of dogs was studied. Isoproterenol (I) resulted in an increase in production of 3′,5′-adenosine monophosphate (cAMP) in these cells. Theophylline (T) did not significantly elevate cAMP levels in the RC when these levels were compared with those of frozen control cells, but when the levels of T-treated cells were compared with the normal decline of cAMP in untreated, incubated RC, the results showed that T was effective in inhibition of the decline of cAMP, presumably due to its activity as a phosphodiesterase inhibitor. I and T together resulted in higher levels of cAMP in RC than either agent alone. I resulted in inhibition of ragweed-induced histamine release from the RC of ragweed-sensitive dogs. Concanavalin A reduced the level of cAMP in RC and resulted in increased release of histamine from cells which was not potentiated by antigen. Levels of cAMP in RC appeared to correlate with the number of macrophages. This does not imply that other RC do not contain cAMP, but that respiratory macrophages may have a higher cAMP content than other types of cells found free in the bronchi.

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