Isolation of 3β, 7α-Dihydroxychol-5-Enoic Acid, an Intermediate of Chenodeoxycholic Acid Biogenesis, and 3α, 7α-Dihydroxychol-4-Enoic Acid from Bladder Bile of Hens
- 1 March 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 83 (3) , 799-805
- https://doi.org/10.1093/oxfordjournals.jbchem.a131975
Abstract
Two Lifschütz-positive C24-bile acids were isolated from bladder bile of hens. One of these was identified by isotope dilution experiments after conversion to a 3H-labeled compound, and also by GLC after methoxylation, as 3β, 7α-dihydroxychol-5-enoic acid, a key intermediate of chenodeoxycholic acid biogenesis. The other, to which the structure 3β, 7α-dihydroxychol-4-enoic acid had been assigned previously, was proved to be its 3α-epimer by several experiments. These findings favor the alternative pathway of chenodeoxycholic acid biogenesis proposed by Yamasaki and his associates.This publication has 1 reference indexed in Scilit: