Quantitative Studies of the Specificity of Anti-Pneumococcal Antibodies, Types III and VIII

Open Access
Publisher Website
Google Scholar
Abstract
A method has been described for labeling of oligosaccharides, isolated from partial hydrolysates of Type III pneumococcal polysaccharide, by reduction of the terminal hexose units with tritiated borohydride. Interactions of rabbit anti-S3 antibody and its purified Fab fragments, with purified, labeled and reduced hexasaccharides, (T)3A6 and (T)3D6, derived respectively from S3 by acid and by enzymatic hydrolysis, were studied by equilibrium dialysis. Homogeneous binding was observed in each case with association constants exceeding 105 L/mol. Removal of the Fc fragment followed by reduction and alkylation to 3.6 S fragments did not significantly alter the magnitude of the association constants.

This publication has 0 references indexed in Scilit: